Friday, April 24, 2020

Vaccine INO-4800 | Social distancing | Vaccine for COVID-19 | BioNTech | Recombinant protein | Moderna’s mRNA 1273 | ChAdox1-ncov 19 | Coronavirus treatment | Clinical trial | USA | UK | China


Chinese biotech firm had proved positive results in an animal model and it is the first vaccine development that was tested in rhesus macaques. Sinovac  Biotech, a biotech firm in china gave 2 different doses of vaccine to 8 macaques and after 4 weeks of observation, none of them had the infection. The human trial began and during the animal testing, the best outcome came from the best dose. This company is a well-experienced vaccine maker and they have previously worked with diseases such as hepatitis A and B  and H5N1 influenza. The human clinical trial consist of 144 volunteers, to whom different doses of the vaccine were tested.


6 vaccines have entered the human trial and 77 more are under development. The major developer uses genetic engineering and the other 4 used old inactivation methods of developing.

Cansino's Ad5-nCov  completed Phase I trial

Cansino's Ad5-nCov  Vaccine candidate successfully completed the phase 1 human trial. This organization started enrolling volunteers for the phase 2 trial. It is a DNA based vaccine that used a recombinant protein approach for development. This firm has also already started working for the phase 2 trial of Ebola vaccine (Adenovirus) Ad5-EBOV. This company is planning to enroll 500 patients in Wuhan. 250 – phase I middle dose, 125-low dose, and 125 placebo injection. These are the number of people involved in dosage optimization.

 Johnson& Johnson

They have not yet started any human trail but they have been working with vaccine development with the same vector and signed a huge deal with HHS/BARDA. The phase I trial will not begin until September.  The adenovirus vector needs a correct approach to grow a more viral vector.

Insect cells  to develop recombinant proteins

The big giant Sanofi used their approach of developing vaccine by using insect cells to recombinant antigen proteins. This approach has been already approved for a flu vaccine. This approach is to be combined with GSK’s adjuvant (immune boosters).



 Vaccine INO-4800

Inovio-4800 is a DNA vaccine that is similar to that of the mRNA vaccine. This firm has been working on this for several years and other diseases such as MERS/SARS. They started with 40 volunteers in the US and another challenge is that they are developing a vaccine delivery devices for injecting through the skin.

mRNA based vaccine
BioNTech has a deal with Fudan's work on coronavirus vaccine in china. They have signed up a deal with Pfizer. German regulatory authorities have approved to begin Phase I and II trails. This trail is to enroll 200 volunteers for dosage evaluation.


ChAdox1-ncov 19

Oxford University has completed its phase I trial which had no safety problem and they are, to begin with, 510 volunteers for phase II trial. It is an Adenovirus vector from a chimpanzee that has a similar concept of the Cansino vaccine.


Moderna’s mRNA 1273

This US-based firm is expertise in mRNA therapies. They are in collaboration with NIH. The study will deal with 45 patients phase I trial in Seattle  and larger group at Emory which will have doses of 25,100,250 mg of mRNA in various age groups.

Summary:
  • Inactivated virus vaccine- Wuhan institute for Biological products,  Sinovac’s PicoVacc
  • mRNA based vaccine- BioNTech/Pfizer, Moderna mRNA-1273
  • Adenovirus vaccine- Cansino's Ad5-nCov  , Johnson& Johnson,  Oxford Vaccine(ChAdOx1-nCov 19)
  • DNA Vaccine- Inovio’s INO-4800
  • Recombinant protein- Sanofi/GSK



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